Electrical interaction between neurons in the pigeon isthmo-optic nucleus.

The present study used brain slices to investigate interneuronal communication in the isthmo-optic nucleus in pigeons. Electrical stimulation of the isthmo-optic tract generated a transmembrane potential in isthmo-optic cells that was obtained by subtracting the extracellular potential from the intracellular potential. This transmembrane potential resulted in enhancement of excitability and/or in production of spikes in 42 (63%) cells. In most cases, proximal axons marked in brain slices by Lucifer yellow were too short to reach the stimulation site, indicating that spikes were evoked by electrical field effect or ephaptic interaction produced by nearby cells whose axons were activated by stimulation. Eleven (16%) cells discharged a spikelet, or spike that was abolished by hyperpolarizing current injection leaving a spikelet. Markings of five of these cells all indicated the presence of dye-couplings, each of which consisted of a pair of cells. Fourteen (21%) cells only produced antidromic spikes with a short and constant latency. Four of these cells were marked and their axons passed through the stimulation site, implying that their nearby cells' axons might be cut too short to be electrically stimulated or they were in a sparse-cell area. The present results provide electrophysiological and neuroanatomical evidence that both electrical field effect and electrical coupling may play important roles in interneuronal communication within the pigeon isthmo-optic nucleus. These findings are supported by anatomical arrangement of densely packed cells and their oriented dendrites in this centrifugal nucleus.